Taking place in the middle of London Fashion Week at the city's iconic St. Paul's Cathedral, near where McQueen grew up, at times the service resembled a fashion show as several of the late designer's friends arrived in their finest clothes.
Naomi Campbell turned heads in black feathers and leather, while her fellow supermodel Kate Moss opted for a black leather minidress and tuxedo jacket, which still allowed for plenty of cleavage.
Inside the cathedral Icelandic performer Bjork, dressed in white and wearing angel wings, sang "Gloomy Sunday," a composition associated with suicide.
Wintour, who spoke during the service, remembered McQueen as "a complex and gifted young man."
"His final collection was a battle between dark and light. His was an 18-year career of harnessing his dreams and demons," she said.
"He showed us everything was possible; dreams could become reality. But he has left us with an even more exceptional legacy, a talent that soared like the birds of his childhood above us all."
McQueen, 40, stunned the fashion world by taking his own life in February. The designer, who reportedly took a cocktail of drugs before hanging himself, had been depressed by the recent death of his mother.
He was remembered in March on what would have been his 41st birthday by a number of prominent New York-based designers including Diane von Furstenberg, Michael Kors, Zac Posen and Calvin Klein's Francisco Costa.
The Alexander McQueen label lives on under the creative direction of Sarah Burton.